LeishTag is a sub-project of the LeishGEM project aiming to determine where Leishmania mexicana proteins localise within the cell. Target proteins are those which do not have a Trypanosoma brucei ortholog, or the T. brucei ortholog has less than 30% sequence identity. This complements existing TrypTag data.
We will tag protein-coding genes with mNeonGreen (Shaner et al., 2013) and determine the localisation in both promastigote and amastigote life cycle stages by fluorescence microscopy of live cells. Wherever possible, we will determine the localisation of the protein when tagged at both the N and C terminus.
To support the parasite research community we are making this data rapidly available as it is generated and before publication. The data on this website is therefore a 'first beta' and subject to change.
LeishTag.org contains data from a major and costly project with the aim of providing the cellular localisation of proteins encoded in the Leishmania genome. Our intention is to make the data is available to the community as it is generated. Until the project is complete, data usage terms are based on the Fort Lauderdale agreement, an agreement reached for pre-publication usage of genome data.
Data on LeishTag.org is available immediately and free to use with acknowledgement. However until the project is complete:
To accept these terms and view localisation images and data please click here.